Recombinant:Article Title: Stem cell migration drives lung repair in living mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-SPC Millipore (Millipore Cat# AB3786, RRID: AB_91588) RAGE/AGER R&D (R and D Systems Cat# MAB1179, RRID: AB_2289349) Goat anti-GFP Rockland (Rockland Cat# 600-101-215, RRID: AB_218182) TdTomato Origene (OriGene Cat# TA150125, RRID: AB_2890005) HOPX Sigma (Sigma-Aldrich Cat# HPA030180, RRID: AB_10603770) polyclonal guineapig LAMP3 Synaptic systems (Synaptic Systems Cat# 391 005, RRID: AB_2713987) LEL-DyLight 649 Vector Laboratories Cat# DL-1178 donkey anti-rabbit AF-647 Invitrogen (Thermo Fisher Scientific Cat# A32795, RRID: AB_2762835) anti-rat AF-488 Invitrogen (Thermo Fisher Scientific Cat# A-21208, RRID: AB_2535794) donkey anti-goat AF-555 Invitrogen (Thermo Fisher Scientific Cat# A-21432 (also A21432), RRID: AB_2535853) VECTASHIELD Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Chemicals, peptides, and recombinant proteins DMEM/F-12, no phenol red Gibco Cat# 21041025 Immunosaver Antigen Retriever Electron Microscopy Science Cat# 64142 Fluoromount-G Invitrogen Cat# 00-4959 Antibiotic-Antimycotic (100X) Gibco Cat# 15240062 Tamoxifen Sigma -Aldrich Cat# T5648 PBS Gibco Cat# 20012027 low gelling temperature melting point agarose Sigma Aldrich Cat# A9414 Bleomycin McKesson Corporation Cat# 1129996 Y-27632 dihydrochloride Tocris Cat# 1254 Cdc42/Rac1 GTPase Inhibitor, ML141 Calbiochem Cat# 217708 Corn oil Sigma-Aldrich Cat# C8267 BD Insyte Autoguard Shielded IV Catheters BD Cat# 381423 24 Well glass bottom plate with high performance #1.5 cover glass Cellvis Cat# P241$5HN Insert Strips IOS, cellGrade +, PC, 1.0 mm BrandTech Cat# 782821 CAS block it life technologies Cat# 008120 Experimental models: Organisms/strains Sftpctm1(cre/ERT2)Blh(Sftpc-CreERT2) Jackson Laboratory Cat# 028054 ArpC3fl/fl Kim I. H. et al., 201341 Rosa26R-CAG-lsl-tdTomato (Ai14) Jackson Laboratory Cat# 07914 B6.129(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato, EGFP)Luo/J Jackson Laboratory Cat# 007576 (Continued on next page) Developmental Cell 59, 830–840.e1–e4, April 8, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Imaris for Cell Biologists package Oxford Instruments N/A GraphPad Prism 9 Graph Pad GraphPad Software FIJI (ImageJ) https://github.com/fiji/fiji N/A R 4.2.1 The R Foundation N/A Python 3 Python Software Foundation N/A ImageJ 1.52b https://imagej.nih.gov/ij/index.html N/A Other Compresstome VF-300-0Z Precisionary Instruments Cat# VF-300-0Z MouseStat for PhysioSuite Kent Scientific N/A
Electron Microscopy:Article Title: Stem cell migration drives lung repair in living mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-SPC Millipore (Millipore Cat# AB3786, RRID: AB_91588) RAGE/AGER R&D (R and D Systems Cat# MAB1179, RRID: AB_2289349) Goat anti-GFP Rockland (Rockland Cat# 600-101-215, RRID: AB_218182) TdTomato Origene (OriGene Cat# TA150125, RRID: AB_2890005) HOPX Sigma (Sigma-Aldrich Cat# HPA030180, RRID: AB_10603770) polyclonal guineapig LAMP3 Synaptic systems (Synaptic Systems Cat# 391 005, RRID: AB_2713987) LEL-DyLight 649 Vector Laboratories Cat# DL-1178 donkey anti-rabbit AF-647 Invitrogen (Thermo Fisher Scientific Cat# A32795, RRID: AB_2762835) anti-rat AF-488 Invitrogen (Thermo Fisher Scientific Cat# A-21208, RRID: AB_2535794) donkey anti-goat AF-555 Invitrogen (Thermo Fisher Scientific Cat# A-21432 (also A21432), RRID: AB_2535853) VECTASHIELD Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Chemicals, peptides, and recombinant proteins DMEM/F-12, no phenol red Gibco Cat# 21041025 Immunosaver Antigen Retriever Electron Microscopy Science Cat# 64142 Fluoromount-G Invitrogen Cat# 00-4959 Antibiotic-Antimycotic (100X) Gibco Cat# 15240062 Tamoxifen Sigma -Aldrich Cat# T5648 PBS Gibco Cat# 20012027 low gelling temperature melting point agarose Sigma Aldrich Cat# A9414 Bleomycin McKesson Corporation Cat# 1129996 Y-27632 dihydrochloride Tocris Cat# 1254 Cdc42/Rac1 GTPase Inhibitor, ML141 Calbiochem Cat# 217708 Corn oil Sigma-Aldrich Cat# C8267 BD Insyte Autoguard Shielded IV Catheters BD Cat# 381423 24 Well glass bottom plate with high performance #1.5 cover glass Cellvis Cat# P241$5HN Insert Strips IOS, cellGrade +, PC, 1.0 mm BrandTech Cat# 782821 CAS block it life technologies Cat# 008120 Experimental models: Organisms/strains Sftpctm1(cre/ERT2)Blh(Sftpc-CreERT2) Jackson Laboratory Cat# 028054 ArpC3fl/fl Kim I. H. et al., 201341 Rosa26R-CAG-lsl-tdTomato (Ai14) Jackson Laboratory Cat# 07914 B6.129(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato, EGFP)Luo/J Jackson Laboratory Cat# 007576 (Continued on next page) Developmental Cell 59, 830–840.e1–e4, April 8, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Imaris for Cell Biologists package Oxford Instruments N/A GraphPad Prism 9 Graph Pad GraphPad Software FIJI (ImageJ) https://github.com/fiji/fiji N/A R 4.2.1 The R Foundation N/A Python 3 Python Software Foundation N/A ImageJ 1.52b https://imagej.nih.gov/ij/index.html N/A Other Compresstome VF-300-0Z Precisionary Instruments Cat# VF-300-0Z MouseStat for PhysioSuite Kent Scientific N/A
Blocking Assay:Article Title: Stem cell migration drives lung repair in living mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-SPC Millipore (Millipore Cat# AB3786, RRID: AB_91588) RAGE/AGER R&D (R and D Systems Cat# MAB1179, RRID: AB_2289349) Goat anti-GFP Rockland (Rockland Cat# 600-101-215, RRID: AB_218182) TdTomato Origene (OriGene Cat# TA150125, RRID: AB_2890005) HOPX Sigma (Sigma-Aldrich Cat# HPA030180, RRID: AB_10603770) polyclonal guineapig LAMP3 Synaptic systems (Synaptic Systems Cat# 391 005, RRID: AB_2713987) LEL-DyLight 649 Vector Laboratories Cat# DL-1178 donkey anti-rabbit AF-647 Invitrogen (Thermo Fisher Scientific Cat# A32795, RRID: AB_2762835) anti-rat AF-488 Invitrogen (Thermo Fisher Scientific Cat# A-21208, RRID: AB_2535794) donkey anti-goat AF-555 Invitrogen (Thermo Fisher Scientific Cat# A-21432 (also A21432), RRID: AB_2535853) VECTASHIELD Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200-10 Chemicals, peptides, and recombinant proteins DMEM/F-12, no phenol red Gibco Cat# 21041025 Immunosaver Antigen Retriever Electron Microscopy Science Cat# 64142 Fluoromount-G Invitrogen Cat# 00-4959 Antibiotic-Antimycotic (100X) Gibco Cat# 15240062 Tamoxifen Sigma -Aldrich Cat# T5648 PBS Gibco Cat# 20012027 low gelling temperature melting point agarose Sigma Aldrich Cat# A9414 Bleomycin McKesson Corporation Cat# 1129996 Y-27632 dihydrochloride Tocris Cat# 1254 Cdc42/Rac1 GTPase Inhibitor, ML141 Calbiochem Cat# 217708 Corn oil Sigma-Aldrich Cat# C8267 BD Insyte Autoguard Shielded IV Catheters BD Cat# 381423 24 Well glass bottom plate with high performance #1.5 cover glass Cellvis Cat# P241$5HN Insert Strips IOS, cellGrade +, PC, 1.0 mm BrandTech Cat# 782821 CAS block it life technologies Cat# 008120 Experimental models: Organisms/strains Sftpctm1(cre/ERT2)Blh(Sftpc-CreERT2) Jackson Laboratory Cat# 028054 ArpC3fl/fl Kim I. H. et al., 201341 Rosa26R-CAG-lsl-tdTomato (Ai14) Jackson Laboratory Cat# 07914 B6.129(Cg)-Gt(ROSA) 26Sortm4(ACTB-tdTomato, EGFP)Luo/J Jackson Laboratory Cat# 007576 (Continued on next page) Developmental Cell 59, 830–840.e1–e4, April 8, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Imaris for Cell Biologists package Oxford Instruments N/A GraphPad Prism 9 Graph Pad GraphPad Software FIJI (ImageJ) https://github.com/fiji/fiji N/A R 4.2.1 The R Foundation N/A Python 3 Python Software Foundation N/A ImageJ 1.52b https://imagej.nih.gov/ij/index.html N/A Other Compresstome VF-300-0Z Precisionary Instruments Cat# VF-300-0Z MouseStat for PhysioSuite Kent Scientific N/A
|
Vector Laboratories is a verified supplier 
Vector Laboratories manufactures this product 